Determination of Mollugin in Mongolian Medicine Bariga-13 Powder by HPLC

doi:10.16153/j.1002-7777.2017.02.012

Chinese Pharmaceutical Affairs ›› 2017, Vol. 31 ›› Issue (2) : 178-181. DOI: 10.16153/j.1002-7777.2017.02.012

Technology Research

Gao Limei¹, Zheng Jian²

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Abstract

Objective: To develop a method for determination of mollugin in a prepared Mongolia traditional medicine Bariga-13.Methods: HPLC method was built by using Dikma Dimonsil Diamonsil C18 (250 mm×4.6 mm, 5μm) as column and methanol-water-tetrahydrofuran (527:90:3) as mobile phase. The detection wavelength was set at 391 nm.Results: The calibration curve of Mollugin was linear (r=0.9999) in the range of 5.5-55 μg·mL^-1. The average recovery was 102.24%; RSD was 1.48%.Conclusion: The method is simple, reproducible and reliable to produce good separation results. It can be applied to the quality test of Bariga-13.

Key words

Mongolian medicine; HPLC; Bariga-13; mollugin

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Gao Limei, Zheng Jian. Determination of Mollugin in Mongolian Medicine Bariga-13 Powder by HPLC[J]. Chinese Pharmaceutical Affairs, 2017, 31(2): 178-181 https://doi.org/10.16153/j.1002-7777.2017.02.012

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