The Determination of the Content of N(2)-L-alanyl-L-glutamine in In Vitro Fertilization Medium By UHPLC-MS/MS

doi:10.16153/j.1002-7777.2018.08.014

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Chinese Pharmaceutical Affairs ›› 2018, Vol. 32 ›› Issue (8) : 1097-1101. DOI: 10.16153/j.1002-7777.2018.08.014

Technology Research

The Determination of the Content of N(2)-L-alanyl-L-glutamine in In Vitro Fertilization Medium By UHPLC-MS/MS

Huang Yuanli, Ke Linnan, Wang Chunren

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Abstract

Objective:To establish a method of determining N(2)-L-alanyl -L-glutamine in in vitro fertilization medium by UHPLC-MS/MS and to analyze the content of N(2)-L-alanyl -L-glutamine in fertilization medium for different purposes. Methods:UHPLC-MS/MS was used. The UHPLC separation was performed on a SUPELCO Discovery HS F5-3 column (150 mm×2.1 mm, 3 μm) in a gradient elute mode with acetonitrile and water (both containing 0.1% formic acid) as the mobile phase at a flow rate of 0.35 mL·min^-1. The column temperature was 40℃. MS detection was performed with multiple reaction monitoring (MRM) mode by using negative electro spray ionization. Results and Conclusion:The linearity was achieved in the range of 1.276-12.76μg·mL^-1 for N(2)-L-alanyl -L-glutamine. The average recovery rates were 100.5%-103.0% with RSD less than 1.98%, which indicates that the method is sensitive, rapid, accurate, and specific. It it will lay a basis for the study of quality standards of in vitro fertilization medium.

Key words

in vitro fertilization medium;N(2)-L-alanyl -L-glutamine;UHPLC-MS/MS;quality control

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Huang Yuanli, Ke Linnan, Wang Chunren. The Determination of the Content of N(2)-L-alanyl-L-glutamine in In Vitro Fertilization Medium By UHPLC-MS/MS[J]. Chinese Pharmaceutical Affairs, 2018, 32(8): 1097-1101 https://doi.org/10.16153/j.1002-7777.2018.08.014

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