Objective: To compare the anesthesia effects of SD rats after intramuscular injection and intraperitoneal injection of Zoletil® 50 or Xylazine, and to compare the effects of intraperitoneal injection on hematology, serum biochemical indexes and pathology of main organs. Methods: Intraperitoneal injection of sodium pentobarbital was used as a control group. SD rats were anesthetized by intramuscular injection and intraperitoneal injection of Zoletil® 50 or Xylazine at low, medium and high doses, and the induction time, duration time of anesthesia and recovery time were observed. The rats were anesthetized by intraperitoneal injection of Zoletil® 50 (50 mg·kg-1) and Xylazine (25 mg·kg-1), and their blood samples and main organs were separated after about 20 min, and then the results of hematological test, serum biochemical test, and histopathological examination were compared. Results: There was no significant difference on the anesthetic effect between the two administration methods of Zoletil ®50, while the induction time was shorter and the duration time was longer than that of Xylazine. There was no significant effect on hematology, serum biochemical indexes and histopathologyby intraperitoneal injection of Zoletil® 50 or Xylazine. Conclusion: Compared with Xylazine, the anesthetic effect of Zoletil® 50 on SD rats is much better and Zoletil® 50 can be used in toxicological evaluation studies of rats.
Key words
Zoletil? 50； Xylazine； SD rat； anesthesia； comparative study
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