Objective: To set up a customized statistical software of profi ciency testing evaluation and reference material assignment—PTP/RMP, in order to solve the problems in the statistical evaluation and calculation of profi ciency testing and standard substance for profi ciency testing providers (PTP) and reference material providers (RMP). Methods: The PTP/RMP is set up based on the statistical methods provided by ISO13528 and ISO Guide35 for profi ciency testing and reference material assignment respectively and on the MATLAB platform for interface design and statistical program construction. Results: (1) PTP/RMP software comprehensively and systematically provides statistical content required in profi ciency testing and reference material determination, including analyzing the uniformity, stability, determination of samples and the laboratory capability evaluation using the tested samples. (2) PTP/RMP software runs complex calculation principles and formulas in the background, makes the interface settings concise and intuitive, convenient and fast and gives full consideration to the non-statistical professional characteristics of users; (3) The accuracy and reliability of the PTP/RMP software was verified by comparing the statistical results of the PTP/RMP software with the statistical results in the regulations and the results from the professional statistical software. Conclusion: PTP/RMP software not only provides a professional statistical software that meets the requirements of domestic and foreign regulations on proficiency testing and reference material assignment, but also provides a new scientific evaluation and supervision tool for the statistical analysis of relevant content for regulatory examiners.