Objective: To explore the growth regularity of Mycoplasma pneumoniae in mycoplasma broth medium and Mycoplasma oral in arginine mycoplasma broth medium, compare the eff ects of diff erent airtight ways on the sensitivity of mycoplasma liquid medium, and investigate the storage validity period of prepared liquid basic medium. Methods: The color change unit (CCU) of Mycoplasma pneumoniae and Mycoplasma oral in the diff erent growth stages was measured by the color change unit test, and their growth curve was drawn. The liquid culture medium was prepared by using three different sealed instruments and the sensitivity was tested. The sensitivity of prepared liquid basic medium was tested at 0, 15, 30, 60 and 90 days. Results: The sensitivity of both mycoplasma broth medium and arginine mycoplasma broth medium reached 109 CCU·mL-1 in tightly stoppered containers with three kinds of instruments. The validity period of mycoplasma broth and arginine mycoplasma broth basic medium was 90 days at 2-8 ℃. Conclusion: This experiment provided a reference for the test and study of the sensitivity of mycoplasma liquid medium.
Dong Jiaojiao, Yang Jiangwei, Zhao Zhuo, Wang Jinheng
. Growth Analysis of Quality Control Strains in Mycoplasma Liquid Culture Medium[J]. Chinese Pharmaceutical Affairs, 2023
, 37(2)
: 210
-214
.
DOI: 10.16153/j.1002-7777.2023.02.013
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