Objective: To establish two methods for the determination of Tribulusteristussaponin K in tribulus teristus saponin extract with CAD and ELSD detectors. Methods: HPLC was performed on Waters Symmetry Shield RP18 column with mobile phase composed of acetonitrile-water at a fl ow rate of 1.0 mL·min-1 in gradient elution mode, the column temperature was 25℃. CAD and ELSD detectors were respectively used to detect, and the content determination results of the samples were compared. Results: The linear relationship between the two methods was good, and the precision, repeatability, stability and recovery rate met the methodological requirements. The detection limit and quantitation limit of CAD were lower than those of ELSD. Conclusion: Both methods can be used to determine the content of Tribulusterrestrissaponin K in tribulus terrestris saponin extract. CAD method has higher sensitivity and precision than ELSD method.
Li Hailiang, Li Yaolei, Zhang Zian, Wang Ying, Jin Hongyu, Ma Shuangcheng
. Comparative Study on Content Determination of Tribulus Terrestris Saponin K in Tribulus Teristus Saponin Extracts by HPLC-CAD and ELSD[J]. Chinese Pharmaceutical Affairs, 2023
, 37(6)
: 680
-686
.
DOI: 10.16153/j.1002-7777.2023.06.007
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