Objective: To determine 278 kinds of pesticides and metabolites in Chrysanthemum, screen the pesticide indicators that should be monitored in Chrysanthemum, so as to provide references for the formulation of maximum residue limits of pesticides in Chrysanthemum, and carry out the dietary exposure assessment of the detected pesticides, so as to provide references for the safe drug use in Chrysanthemum. Methods: A total of 278 indicators of banned, commonly used pesticides and metabolites with toxicological significance in 78 batches of Chrysanthemum were detected by gas chromatography-mass spectrometry (GC-MS/MS) and liquid chromatography-mass spectrometry (LC-MS/MS); the acute and chronic risks of highly detected pesticides (the detection rate was more than 15%) in Chrysanthemum were evaluated by point assessment, and the risk ranking matrix was used to calculate and rank the risk scores of highly detected pesticides. Results: 71 pesticides were detected in 78 batches of Chrysanthemum, including 3 banned pesticides (phorate, carbofuran and fl ufenitrile), of which 24 pesticides were detected with a rate of more than 15.0%. The acute and chronic dietary intake risks of pesticides with high detection rate were acceptable. According to the risk score, 24 pesticides could be divided into 3 categories, including 4 high-risk pesticides, 7 medium-risk pesticides and 13 low-risk pesticides. Conclusion: Banned pesticides are detected in some batches of Chrysanthemums. It is necessary and timely to increase the inspection of banned pesticide residues to ensure the medicinal safety of Chrysanthemum in the Pharmacopoeia of the People's Republic of China (2020 Edition). There are many kinds of pesticides varieties detected in Chrysanthemum. It is suggested to speed up the registration of pesticides varieties actually used in Chrysanthemum planting in order to standardize production.