Objective: To conduct a basic identification study of folk Ding medicine herb samples in Yunnan and Guizhou regions with DNA barcoding technology, based on the pharmacognosy identification and psbA- trnH sequences. Methods: By collecting the samples of Ding medicine herb, the key identification features were screened out by using botanical trait analysis, microscopic observation, and thin-layer chromatography (TLC) technique. Meanwhile, the total genomic DNA of the samples was extracted, and specific primers were designed to amplify the gene sequences of Ding medicine based on psbA-trnH sequences, after sequencing, splicing and proofreading, the species origin was identified by BLAST comparison in the NCBI database. Results: The original botanical form of Ding medicine, the microscopic features of powdered and dissociated tissues of the herb were obvious, and its thin-layer chromatography showed exclusive spots that possessed effective identification value. Further psbA-trnH sequence analysis showed that the psbA-trnH sequence similarity between Ding medicine and Orixa japonica Thunb. was extremely high, reaching 99.80%. Conclusion: Based on the pharmacognosy characteristics and psbA-trnH sequences as DNA barcodes, it is possible to identify the basal plant of Ding medicine as Orixa japonica Thunb. which provides a reference to ensure the scientific identification of the source of Ding medicine in Yunnan and Guizhou regions.
Qin Li
,
Luo Zhengcai
,
Chen Qirun
,
Hu Yilan
,
Zhang Xu
. Identification of Ding Medicine based on Pharmacognosy and DNA Barcoding[J]. Chinese Pharmaceutical Affairs, 2024
, 38(11)
: 1287
-1294
.
DOI: 10.16153/j.1002-7777.20240524
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