Objective: To establish a qualitative and quantitative method for quality control of Spilanthes paniculata Wall. ex DC., and investigate the quality of this medicine in Guangxi at different habitats and harvest time. Methods: The TLC method was used for qualitative identification with butyl acetate-ethyl acetate-formic acid-water (11:3:1.5:1.5) being developing agent. The content determination of chlorogenic acid was conducted by HPLC by using a C18 column (4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.4% phosphoric acid (10:90) at a flow rate of 1.0 mL·min^-1, the detection wavelength was 327 nm, and the column temperature was 30℃. Results: The spot of chlorogenic acid could be well identified by TLC. In the system of HPLC, chlorogenic acid showed good linear relationship in the range of 2.032-25.40 μg·mL^-1(r=0.9995). The recovery rate was 100.9% (n=6), RSD=1.2%. The contents of the 13 batches of samples were between 0.042 and 0.741 mg·g^-1. Conclusion: The method is simple, accurate, reliable and can be used for the quality control of Spilanthes paniculata Wall. ex DC.