冬虫夏草人工繁育品与野生品基于甾醇特征图谱的比较研究

doi:10.16153/j.1002-7777.2017.02.020

中国药事 ›› 2017, Vol. 31 ›› Issue (8) : 951-959. DOI: 10.16153/j.1002-7777.2017.02.020

药物研究

过立农¹, 刘杰¹, 马双成¹, 昝珂¹, 郑健¹, 张美², 钱正明³, 李文佳³

作者信息 +

Comparative Study of Characteristic Chromatogram of Cultivated and Wild Cordyceps sinensis Based on Sterols

Guo Linong¹, Liu Jie¹, Ma Shuangcheng¹, Zan Ke¹, Zheng Jian¹, Zhang Mei², Qian Zhengming³, Li Wenjia³

Author information +

文章历史 +

摘要

目的：对冬虫夏草人工繁育品与野生品进行比较研究，建立基于甾醇类成分的特征图谱，并比较麦角甾醇含量的差异，为该药材的质量监管提供参考。方法：以10批冬虫夏草人工繁育品、17批野生品以及5批凉山虫草为研究对象，采用超高效液相色谱法，以麦角甾醇为对照品，选用XBridgeC18色谱柱（100 mm×2.1 mm，1.6 μm）；流动相为甲醇-水（95：5），检测波长282 nm，流速0.3 mL·min^-1，柱温30℃，采用CHEMPATTERN化学计量学软件对野生品色谱图进行处理，生成特征图谱，并计算所有批次的相似度。结果：17批野生冬虫夏草、10批冬虫夏草人工繁育品与野生品对照图谱之间的相似度均大于0.9，相似度良好；而5批凉山虫草相似度均未超过0.8，相似度较低。10批人工繁育品的平均含量为0.121%±0.031%；17批野生品的平均含量为0.115%±0.045%；5批凉山虫草的平均含量为0.468%±0.038%。冬虫夏草人工繁育品和野生品基于甾醇的特征图谱以及麦角甾醇含量基本一致；与凉山虫草有显著性差异。结论：本研究有助于冬虫夏草的真伪鉴别，同时为冬虫夏草人工繁育资源开发以及合理使用提供科学依据。

Abstract

Objective: To establish the UPLC characteristic chromatogram based on sterols and to compare the differences of the contents of ergosterol in cultivated and wild Cordyceps sinensis so as to provide references for the supervision of this crude drug. Methods: 10 batches of cultivated Cordyceps sinensis, 17 batches of wild Cordyceps sinensis and 5 batches of Cordyceps liangshanensis were used as objects and ergosterol was used as reference substance. UPLC analysis was performed on a Waters XBridge C18 column (100×2.1 mm; 1.6 μm). The mobile phase was methanol-water (95:5) with isocratic elution. The flow rate was 0.3 mL.min^-1 and the detection wavelength was 282 nm and the column temperature was 30。C. Chemometrics software Chempattern was employed to generate characteristic chromatogram and calculate similarity. Results: Similarities of 17 batches of wild Cordyceps sinensis and 10 batches of cultivated Cordyceps sinensis were more than 0.9, indicating good similarity. But the similarities of 5 batches of Cordyceps liangshanensis were no more than 0.8, indicating poor similarity. The average contents of ergosterol were 0.121% ±0.031% in cultivated Cordyceps sinensis, 0.115% ±0.045% in wild Cordyceps sinensis, and 0.468% ±0.038% in Cordyceps liangshanensis. UPLC characteristic chromatogram and content of ergosterol in cultivated Cordyceps sinensis were basically identical to those of the wild Cordyceps sinensis and showed significant differences from adulterant Cordyceps liangshanensis. Conclusion: The results were helpful to authenticate Cordyceps sinensis and could provide scientific references for the utilization of cultivated Cordyceps sinensis, and the resource development of chinese crude dyug.

导出引用

过立农, 张美, 刘杰, 马双成, 钱正明, 李文佳, 昝珂, 郑健. 冬虫夏草人工繁育品与野生品基于甾醇特征图谱的比较研究[J]. 中国药事, 2017, 31(8): 951-959 https://doi.org/10.16153/j.1002-7777.2017.02.020

Guo Linong, Zhang Mei, Liu Jie, Ma Shuangcheng, Qian Zhengming, Li Wenjia, Zan Ke, Zheng Jian. Comparative Study of Characteristic Chromatogram of Cultivated and Wild Cordyceps sinensis Based on Sterols[J]. Chinese Pharmaceutical Affairs, 2017, 31(8): 951-959 https://doi.org/10.16153/j.1002-7777.2017.02.020

中图分类号： R917 R284.1

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