目的：建立一测多评法(QAMS)同时测定血塞通片中三七皂苷R₁、人参皂苷Rg₁、人参皂苷 Re、人参皂苷Rb₁、人参皂苷Rd 5种成分的含量。方法：采用高效液相法，使用Waters Symmetry Shield RP₁₈色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-水溶液为流动相，梯度洗脱，流速1.3 mL·min^-1， 柱温25 ℃，检测波长为203 nm。以人参皂苷Re为内标参照物，建立其与三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb₁、人参皂苷Rd的相对校正因子(RCFs)；通过一测多评法与外标法(ESM)结果的比较， 验证所建立方法的可行性和准确性。结果：5种皂苷在一定范围的浓度内呈良好的线性关系；三七皂苷 R₁、人参皂苷Rg₁、人参皂苷Rb₁、人参皂苷Rd的相对校正因子分别为0.996、0.856、1.165、0.996；且在不同试验条件下重现性良好(RSD＜3.0%)。结论：建立了血塞通片中5种成分的一测多评法，经方法学验证，该法可用于血塞通片中5种皂苷成分的含量测定。

Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) for the simultaneous determination of five components (Notoginsenoside R₁, Ginsenoside Rg₁, Ginsenoside Re, Ginsenoside Rb₁, Ginsenoside Rd) in Xuesaitong tablets. Methods: HPLC was performed on Waters Symmetry Shield RP₁₈ column (4.6 mm×250 mm, 5 μm) at 25 ℃, with mobile phase composed of acetonitrile-water at a flow rate of 1.3 mL·min_-1 in gradient elution mode, and the detection wavelength was set at 203 nm. Using Ginsenoside Re as the internal reference substance, the relative correlation factors (RCFs) of Notoginsenoside R₁, Ginsenoside Rg₁, Ginsenoside Rb₁ and Ginsenoside Rd were calculated. By comparing the results of the external standard method (ESM) and QAMS methods, the feasibility and accuracy of QAMS method were verified. Results: The standard curves of five Saponins had good linear relationship in the ranges of the tested concentrations. The RCFs of Notoginsenoside R₁, Ginsenoside Rg₁, Ginsenoside Rb₁ and Ginsenoside Rd were0.996, 0.856, 1.165 and 0.996, respectively, and the reproducibility was good under different experimental conditions (RSD＜3.0%). Conclusion: The method of QAMS for five Saponins in Xuesaitong tablets was established. The validated method could be used for the assay of 5 Saponins in Xuesaitong tablets.