目的：建立养阴清肺丸中同时测定甲基麦冬黄烷酮A、甲基麦冬黄烷酮B、山麦冬皂苷B和短葶山麦冬皂苷C的分析方法，判断麦冬的投料情况。方法：应用高效液相色谱-三重四极杆串联质谱 (HPLC-MS/MS)技术，采用Agilent Poroshell 120 EC-C₁₈(3.0 mm×100 mm，2.7μm)色谱柱， 以乙腈-0.1%甲酸溶液为流动相，流速0.3 mL·min^-1，柱温35 ℃，进样量5 μL；采用电喷雾离子源 (ESI)，正、负离子2种模式，以多反应监测模式(MRM)进行分析。结果：4个成分在相应的范围内线性关系良好(r＞0.9991)，平均回收率为96.61% ~98.65%。59批养阴清肺丸样品中有39批存在以山麦冬替代或者混入麦冬投料问题。结论：建立的方法简单、准确、可靠，可用于判断养阴清肺丸中麦冬的投料情况及市场监管。

Objective: To establish a method for the simultaneous determination of Methylophiopogonanone A, Methylophiopogonanone B, Liriopesides B and Liriopemuscari baily saponins C in Yangyin Qingfei pills and determine the feeding situation of Ophiopogonis Radix. Methods: The high performance liquid chromatographytriple quadruple mass spectrometry (HPLC-MS/MS) was performed on an Agilent Poroshell 120 EC-C₁₈ column (3.0 mm × 100 mm, 2.7 μm) with mobile phase consisting of acetonitrileand 0.1% aqueous formic acid. The fl ow rate was controlled at 0.3 mL•min^-1, and the column temperature was set at 35 ℃, and the sample volume was 5 μL. A mass spectrometer equipped with electrospray ionization source was used as detector operated in positive and negative ion mode. Multiple reaction monitoring (MRM) was performed. Results: Four components showed good linear correlation within their ranges (r＞0.9991), and the averaged recoveries ranged from 96.61% to 98.65%. In the 59 batches of Yangyin Qingfei pills, 39 batches had the feeding problem of using Liriopes Radix instead of Ophiopogonis Radix or mixing in Ophiopogonis Radix. Conclusion: The established method is simple, accurate, reliable, and could be used in the determination of the feeding situation of Ophiopogonis Radix in Yangyin Qingfei pills and market regulation.