常用“蒂达”类藏药材四数獐牙菜的成分分析

苏媛, 刘安平, 刘学良, 李维业, 韩天楚, 杨增亮, 王开祥, 刘海青

中国药事 ›› 2022, Vol. 36 ›› Issue (11) : 1280-1285.

中国药事 ›› 2022, Vol. 36 ›› Issue (11) : 1280-1285. DOI: 10.16153/j.1002-7777.2022.11.010
监管技术

常用“蒂达”类藏药材四数獐牙菜的成分分析

  • 苏媛1, 刘安平1, 李维业1, 杨增亮1, 王开祥1, 刘学良2, 韩天楚2, 刘海青2
作者信息 +

Component Analysis of Swertiatetraptera

  • Su Yuan1, Liu Anping1, Li Weiye1, Yang Zengliang1, Wang Kaixiang1, Liu Xueliang2, Han Tianchu2, Liu Haiqing2
Author information +
文章历史 +

摘要

目的:建立高效液相色谱法同时测定四数獐牙菜中獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基三酮含量的方法。方法:采用Waters SunFire C18柱(4.6 mm×250 mm, 5 µm);以0.05%磷酸溶液-甲醇为流动相进行梯度洗脱;柱温30 ℃,流速1.0 mL·min-1,检测波长254 nm。结果:獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基三酮分别在 0.0320~0.3202μg(r=0.9997)、0.3788~3.7881μg(r=0.9998)、0.0124~0.1237μg(r=0.9995)、 0.0032~0.0321μg(r=0.9998)、0.0031~0.0308μg(r=0.9994)进样质量范围内呈良好的线性关系, 加样回收率分别为98.69%、97.81%、92.21%、93.44%、92.77%,RSD(r=6)分别为0.76%、1.77%、 1.98%、1.59%、1.79%。7批样品中獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基三酮的测定结果分别为0.1924%~0.3740%、2.1426%~4.4514%、0.1122%~0.1973%、0.0286%~0.0339%及 0.0043%~0.0938%。结论:该方法简便、高效、灵敏、准确、经济实用,具有良好的重复性和稳定性, 对于四数獐牙菜的质量控制、综合利用提供了有效参考依据。

Abstract

Objective: To establish a HPLC method for the simultaneous determination of Swertiamarin, Gentiopicrin, Sweroside, Dangyao alcohol glycoside and 1-hydroxy-3,4,5 trimethoxydione in Swertiatetraptera. Methods: Waters Sunfire C18 column (4.6 mm × 250 mm, 5 µm) was used; gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase; the column temperature was 30 ℃, the flow rate was 1.0 mL·min-1, and the detection wavelength was 254 nm. Results: Five components have shown good linear relationship within the injection quality ranges of 0.0320-0.3202 μg (r=0.9997), 0.3788-3.7881 μg (r=0.9998), 0.0124-0.1237 μg (r=0.9995), 0.0032-0.0321 μg (r=0.9998) and 0.0031-0.0308 μg (r=0.9994) respectively. The recovery rates were 98.69%, 97.81%, 92.21%, 93.44% and 92.77% (n=6), and RSDs (r=6) were 0.76%, 1.77%, 1.98%, 1.59% and 1.79%. The results of the determination of the five components in 7 batches of samples were 0.1924%-0.3740%, 2.1426%-4.4514%, 0.1122%-0.1973%, 0.0286%-0.0339% and 0.0043%-0.0938% respectively. Conclusion: The method is simple, efficient, sensitive, accurate, economical and practical with good repeatability and stability. It provides an effective reference for the quality control and comprehensive utilization of Swertiatetraptera.

关键词

四数獐牙菜;高效液相色谱法;獐牙菜苦苷;龙胆苦苷;当药苷;当药醇苷;1-羟基-3;4;5三甲氧基三酮;含量测定

Key words

Swertiatetraptera; HPLC; Swertiamarin; Gentiopicrin; Sweroside; Dangyao alcohol glycoside; 1-hydroxy-3;4;5 trimethoxydione; content determination

引用本文

导出引用
苏媛, 刘安平, 刘学良, 李维业, 韩天楚, 杨增亮, 王开祥, 刘海青. 常用“蒂达”类藏药材四数獐牙菜的成分分析[J]. 中国药事, 2022, 36(11): 1280-1285 https://doi.org/10.16153/j.1002-7777.2022.11.010
Su Yuan, Liu Anping, Liu Xueliang, Li Weiye, Han Tianchu, Yang Zengliang, Wang Kaixiang, Liu Haiqing. Component Analysis of Swertiatetraptera[J]. Chinese Pharmaceutical Affairs, 2022, 36(11): 1280-1285 https://doi.org/10.16153/j.1002-7777.2022.11.010

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青海省基础研究项目(编号 2020-ZJ747)

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