Su Yuan1, Liu Anping1, Li Weiye1, Yang Zengliang1, Wang Kaixiang1, Liu Xueliang2, Han Tianchu2, Liu Haiqing2
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(1)Xining Center for Food and Drug Inspection & Testing, Xining 810007 , China; (2)Qinghai Center for Drug Evaluation and Verification, Xining 810007 , China
Objective: To establish a HPLC method for the simultaneous determination of Swertiamarin, Gentiopicrin, Sweroside, Dangyao alcohol glycoside and 1-hydroxy-3,4,5 trimethoxydione in Swertiatetraptera. Methods: Waters Sunfire C18 column (4.6 mm × 250 mm, 5 µm) was used; gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase; the column temperature was 30 ℃, the flow rate was 1.0 mL·min-1, and the detection wavelength was 254 nm. Results: Five components have shown good linear relationship within the injection quality ranges of 0.0320-0.3202 μg (r=0.9997), 0.3788-3.7881 μg (r=0.9998), 0.0124-0.1237 μg (r=0.9995), 0.0032-0.0321 μg (r=0.9998) and 0.0031-0.0308 μg (r=0.9994) respectively. The recovery rates were 98.69%, 97.81%, 92.21%, 93.44% and 92.77% (n=6), and RSDs (r=6) were 0.76%, 1.77%, 1.98%, 1.59% and 1.79%. The results of the determination of the five components in 7 batches of samples were 0.1924%-0.3740%, 2.1426%-4.4514%, 0.1122%-0.1973%, 0.0286%-0.0339% and 0.0043%-0.0938% respectively. Conclusion: The method is simple, efficient, sensitive, accurate, economical and practical with good repeatability and stability. It provides an effective reference for the quality control and comprehensive utilization of Swertiatetraptera.
Su Yuan, Liu Anping, Liu Xueliang, Li Weiye, Han Tianchu, Yang Zengliang, Wang Kaixiang, Liu Haiqing.
Component Analysis of Swertiatetraptera[J]. Chinese Pharmaceutical Affairs, 2022, 36(11): 1280-1285 https://doi.org/10.16153/j.1002-7777.2022.11.010