中国药事 >

2024 , Vol. 38 >Issue 11: 1323 - 1337

DOI: https://doi.org/10.16153/j.1002-7777.20240098

研究进展

枸杞子化学成分及质量评价研究进展

  • 罗群 ,
  • 王莹 ,
  • 李耀磊 ,
  • 刘芫汐 ,
  • 王丹丹 ,
  • 金红宇 ,
  • 马双成 ,
  • 魏锋
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  • 1.沈阳药科大学,沈阳 117004;
    2.中国食品药品检定研究院,北京 102629;
    3.国家药典委员会,北京 100061
罗群 Tel：（010）53852484;E-mail：3038729683@qq.com,共同第一作者：王莹 Tel：（010）53852484;E-mail：wayi_1986@163.com
金红宇 Tel：（010）53852484;E-mail：jhyu@nifdc.org.cn;魏锋 Tel：（010）53852020;E-mail：weifeng@nifdc.org.cn

收稿日期: 2024-01-30

  网络出版日期: 2025-02-05

基金资助

国家自然基金委员会青年基金项目（编号 82204617）,国家重点研发计划项目（编号 2022YFC3501505）

收起

Progress of Chemical Composition and Quality Evaluation of Lycium barbarum L.

  • Luo Qun ,
  • Wang Ying ,
  • Li Yaolei ,
  • Liu Yuanxi ,
  • Wang Dandan ,
  • Jin Hongyu ,
  • Ma Shuangcheng ,
  • Wei Feng
Expand
  • 1. Shenyang Pharmaceutical University, Shenyang 117004, China;
    2. National Institutes for Food and Drug Control, Beijing 102629, China;
    3. Chiness Pharmacopoeia Committee, Beijing 100061, China

Received date: 2024-01-30

  Online published: 2025-02-05

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摘要

目的: 为枸杞子质量评价模式的构成提供思路,奠定基础。方法: 基于近年来的文献资料,对枸杞子的化学成分、质量影响因素、现有标准及质量评价模式研究进展等梳理归纳。结果与结论: 枸杞子活性成分种类复杂,包括枸杞多糖、类胡萝卜素、黄酮和维生素等,质量受产地、品种、种植加工方式等因素的影响。枸杞子药典标准主要以甜菜碱和枸杞多糖作为质控指标,而现有商品规格等级划分中主要以枸杞子果粒大小作为评价依据,均具有一定的局限性。现已有的枸杞子质量评价研究通过灰色关联度分析法、层次分析法和判别分析法等探索枸杞子多个质量参数的权重系数,进而建立质量评价模型,对枸杞子质量进行综合评分。为建立科学、统一的枸杞子质量评价标准提供思路。

关键词： 枸杞子; 化学成分; 质量评价; 等级划分; 多糖

本文引用格式

罗群 , 王莹 , 李耀磊 , 刘芫汐 , 王丹丹 , 金红宇 , 马双成 , 魏锋 . 枸杞子化学成分及质量评价研究进展[J]. 中国药事, 2024 , 38(11) : 1323 -1337 . DOI: 10.16153/j.1002-7777.20240098

Abstract

Objective: To provide ideas and lay the foundation for the composition of quality evaluation model of Lycium barbarum L.. Methods: Based on the literature in recent years, the chemical composition, quality influencing factors, existing standards and the research progress of quality evaluation model of L. barbarum L. were reviewed and summarized. Results and Conclusion: The types of active ingredients of L. barbarum L. were complex, including lycium barbarum polysaccharides, carotenoids, flavonoids and vitamins, etc., and the quality was affected by factors such as place of origin, species, planting and processing methods. The pharmacopoeia standard of L. barbarum L. mainly uses betaine and lycium barbarum polysaccharide as quality control indicators, while the existing commodity specification grade division mainly uses the fruit size as the evaluation basis, which has certain limitations. The existing researches on quality evaluation of L. barbarum L. explored the weight coefficients of multiple quality parameters of L. barbarum L. through gray correlation analysis, hierarchical analysis and discriminant analysis, etc., and then established a quality evaluation model to score the quality of L. barbarum L. comprehensively. Provide ideas for the establishment of scientific and unified quality evaluation standard of L. barbarum L..

Key words： Lycium barbarum L.; chemical composition; quality evaluation; grade division; polysaccharides

参考文献

[1] 徐常青,刘赛,徐荣,等. 我国枸杞主产区生产现状调研及建议[J]. 中国中药杂志,2014,39(11):1979-1984.
[2] 中华人民共和国药典:一部[S]. 2020.
[3] 谢忱,徐丽珍,李宪铭,等. 枸杞子化学成分的研究[J]. 中国中药杂志,2001(5): 35-36.
[4] 罗琼,阎俊,张声华. 枸杞多糖的分离纯化及其抗疲劳作用[J]. 卫生研究,2000(2):53-55.
[5] Yang T, Hu Y, Yan Y, et al.Characterization and Evaluation of Antioxidant and Anti-Inflammatory Activities of Flavonoids from the Fruits of Lycium barbarum[J]. Foods, 2022, 11(3):306.
[6] 孟协中,胡向群,张桂兰,等. 枸杞子和枸杞叶化学成分的研究——第2报杞枸子和杞枸叶中的氨基酸[J]. 中药通报,1987(5):44-46.
[7] 魏雪松,王海洋,孙智轩,等. 宁夏枸杞化学成分及其药理活性研究进展[J]. 中成药,2018,40(11): 2513-2520.
[8] Wei J, Zhang L, Liu J, et al.Protective Effect of Lycium barbarum Polysaccharide on Ethanol-Induced Injury in Human Hepatocyte and its Mechanism[J]. Journal of Food Biochemistry,2020,44(10):e13412.
[9] 李菁菁,罗琼,闫俊,等. 枸杞多糖对糖尿病肾病兔肾功能及MCP-1ICAM-1mRNA表达的影响[J]. 营养学报,2015,37(1):32-36,40.
[10] 邹俊华,梁红业,闵凌峰,等. 枸杞子的抗衰老功效及增强DNA修复能力的作用[J]. 中国临床康复,2005 (11):132-133.
[11] 胡馨予,赵冰,孙晓琪,等. 枸杞子多糖抗疲劳活性研究[J]. 食品科技,2015,40(7):197-200.
[12] 田阳,龚桂萍,路宇,等. 枸杞子多糖不同组分的双向免疫调节机制研究进展[J]. 食品科学,2022,43(23):356-366.
[13] Zhu Y, Zhao Q, Gao H, et al.Lycium barbarum Polysaccharides Attenuates N-methy-N-nitrosourea-induced Photoreceptor Cell Apoptosis in Rats Through Regulation of Poly(ADP-ribose)Polymerase and Caspase Expression[J]. Journal of Ethnopharmacology, 2016,191:125-134.
[14] MAXIMIZE MARKET RESEARCH. GojiBerries Market-Global Industry Analysis and Forecast(2024-2030) [EB/OL].(2024-06)[2024-07-30]. https://www.maximizemarketresearch.com/market-report/global-goji-berries-market/71727/.
[15] 王益民,张珂,许飞华,等. 不同品种枸杞子营养成分分析及评价[J]. 食品科学,2014,35(1):34-38.
[16] 杨晓萍,张声华. 枸杞子糖类的研究[J]. 林产化学与工业,1998(2):65-68.
[17] 邱树磊,胡国柱,陈玉库,等. 枸杞多糖的提取纯化及体内外抗氧化活性研究[J]. 动物医学进展,2021,42(10):56-61.
[18] 缪凤. 枸杞多糖酶提、分离纯化及抗氧化和免疫活性研究[D]. 扬州大学,2021.
[19] 牛忻,闫娜,李笃信,等. 枸杞多糖的分离鉴定与体外肠道代谢初步分析[J]. 天然产物研究与开发,2019, 31(10):1682-1687,1730.
[20] 王生琳,宰清勇,陈华国,等. 5种枸杞多糖生物活性的比较研究[J]. 中国中药杂志,2024,49(1):110-122.
[21] 甘璐. 枸杞多糖抗肿瘤机理研究—细胞凋亡、相关因子基因表达[D]. 武汉:华中农业大学, 2004.
[22] Cao Y, Kou R, Huang X, et al.Separation of Polysaccharides from Lycium barbarum L. by High-Speed Countercurrent Chromatography with Aqueous Two-Phase System[J]. International Journal of Biological Macromolecules, 2024,256:128282.
[23] Zeng W, Chen L, Xiao Z, et al.Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of Lycium barbarum Polysaccharides[J]. Molecules,2023,28(2):701.
[24] Zhou W, Kan X, Chen G, et al.The Polysaccharides from the Fruits of Lycium barbarum L. Modify the Gut Community Profile and Alleviate Dextran Sulfate Sodium-Induced Colitis in Mice[J]. International Journal of Biological Macromolecules,2022,222:2244-2257.
[25] Wang H, Li Y, Dai Y, et al.Screening, Structural Characterization and Anti-Adipogenesis Effect of a Water-Soluble Polysaccharide from Lycium barbarum L. by an Activity-Oriented Approach[J]. Food Bioscience,2023, 53:102502.
[26] Liu J, Li Y, Pu Q, et al.A Polysaccharide from Lycium barbarum L.:Structure and Protective Effects Against Oxidative Stress and High-Glucose-Induced Apoptosis in ARPE-19 Cells[J]. International Journal of Biological Macromolecules, 2022, 201: 111-120.
[27] Zhou L, Liao W, Chen X, et al.An Arabinogalactan from Fruits of Lycium barbarum L. Inhibits Production and Aggregation of Aβ42[J]. Carbohydrate Polymers,2018, 195:643-651.
[28] Gong G, Dang T, Deng Y, et al.Physicochemical Properties and Biological Activities of Polysaccharides from Lycium barbarum Prepared by Fractional Precipitation[J]. International Journal of Biological Macromolecules, 2018,109:611-618.
[29] Zhang Q, Lv X, Wu T, et al.Composition of Lycium barbarum Polysaccharides and Their Apoptosis-Inducing Effect on Human Hepatoma SMMC-7721 Cells[J]. Food & Nutrition Research,2015,59(1):28696.
[30] Liu W. Structure Characterization, Chemical and Enzymatic Degradation, and Chain Conformation of An Acidic Polysaccharide from Lycium barbarum L.[J]. Carbohydrate Polymers,2016,147:114-124.
[31] Tang HL, Chen C, Wang SK, et al.Biochemical Analysis and Hypoglycemic Activity of a Polysaccharide Isolated from the Fruit of Lycium barbarum L.[J]. International Journal of Biological Macromolecules,2015,77:235-242.
[32] Zhang X, Li Y, Cheng J, et al.Immune Activities Comparison of Polysaccharide and Polysaccharide-Protein Complex from Lycium barbarum L.[J]. International Journal of Biological Macromolecules,2014,65:441-445.
[33] Zhu J, Liu W, Yu J, et al.Characterization and Hypoglycemic Effect of a Polysaccharide Extracted from the fruit of Lycium barbarum L.[J]. Carbohydrate Polymers, 2013,98(1):8-16.
[34] Ke M, Zhang XJ, Han ZH, et al.Extraction, Purification of Lycium barbarum Polysaccharides and Bioactivity of Purified Fraction[J]. Carbohydrate Polymers,2011,86(1):136-141.
[35] Yuan Y, Wang Y B, Jiang Y, et al.Structure Identification of a Polysaccharide Purified from Lycium barbarium fruit[J]. International Journal of Biological Macromolecules, 2016,82:696-701.
[36] Wang Z, Liu Y, Sun Y, et al.Structural Characterization of LbGp1 from the Fruits of Lycium barbarum L.[J]. Food Chemistry,2014,159:137-142.
[37] Peng X, Tian G.Structural Characterization of the Glycan Part of Glycoconjugate LbGp2 from Lycium barbarum L.[J]. Carbohydrate Research,2001,331(1): 95-99.
[38] Huang L J, Tian G Y, Ji G Z.Structure Elucidation of Glycan of Glycoconjugate LbGp3 Isolated from the Fruit of Lycium barbarum L.[J]. Journal of Asian Natural Products Research,1999,1(4): 259-267.
[39] 黄琳娟,林颖,田庚元,等. 枸杞子中免疫活性成分的分离、纯化及物理化学性质的研究[J]. 药学学报, 1998(7):33-37.
[40] Yang Y, Chang Y, Wu Y, et al.A Homogeneous Polysaccharide from Lycium barbarum:Structural Characterizations, Anti-Obesity Effects and Impacts on Gut Microbiota[J]. International Journal of Biological Macromolecules,2021,183:2074-2087.
[41] Yao R, Huang C, Chen X, et al.Two Complement Fixing Pectic Polysaccharides from Pedicel of Lycium barbarum L. Promote Cellular Antioxidant Defense[J]. International Journal of Biological Macromolecules,2018,112:356-363.
[42] Ma ZF, Zhang H, Teh SS, et al.Goji Berries as a Potential Natural Antioxidant Medicine:An Insight into Their Molecular Mechanisms of Action[J]. Oxidative Medicine and Cellular Longevity,2019,2019:1-9.
[43] 马亚男. 不同处理方式的枸杞中类胡萝卜素生物可及性与抗氧化性研究[D]. 银川:宁夏大学,2022.
[44] Inbaraj BS, Lu H, Hung CF, et al.Determination of Carotenoids and Their Esters in Fruits of Lycium barbarum Linnaeus by HPLC-DAD-APCI-MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2008,47(4-5):812-818.
[45] 李忠,彭光华,张声华. 非水反相高效液相色谱法分离测定枸杞子中的类胡萝卜素[J]. 色谱,1998(4): 63-65.
[46] 高凯. 宁夏枸杞子的活性成分研究和应用开发[D]. 西安:第四军医大学,2015.
[47] 孙波,杜漠雨,张易木,等. 枸杞鲜果冻干制品中枸杞黄酮的含量及抗氧化活性研究[J]. 时珍国医国药, 2015,26(6):1347-1348.
[48] 莫仁楠,夏园园,曲玮,等. 枸杞子多酚类化合物研究进展[J]. 海峡药学,2016,28(4):1-4.
[49] Qian D, Zhao Y, Yang G, et al.Systematic Review of Chemical Constituents in the Genus Lycium (Solanaceae)[J]. Molecules,2017,22(6):911.
[50] Wu WB, Hung DK, Chang FW, et al.Anti-Inflammatory and Anti-Angiogenic Effects of Flavonoids Isolated from Lycium barbarum Linnaeus on Human Umbilical vein Endothelial Cells[J]. Food & Function,2012,3(10): 1068-1081.
[51] Ali MC, Chen J, Zhang H, et al.Effective Extraction of Flavonoids from Lycium barbarum L. Fruits by Deep Eutectic Solvents-Based Ultrasound-Assisted Extraction[J]. Talanta,2019,203:16-22.
[52] Le K, Chiu F, Ng K.Identification and Quantification of Antioxidants in Fructus lycii[J]. Food Chemistry,2007, 105(1):353-363.
[53] Inbaraj BS, Lu H, Kao TH, et al.Simultaneous Determination of Phenolic Acids and Flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2010,51(3):549-556.
[54] 杨春霞. 高效液相色谱法测定枸杞中9种酚酸化合物含量[J]. 食品研究与开发,2021,42(20):148-153.
[55] 刘少静,刘萌,杨黎彬. RP-HPLC法同时测定枸杞中4种酚酸类成分的含量[J]. 应用化工,2016,45(6): 1181-1183.
[56] 黄钰馨,马玲,李苗,等. 枸杞子中甜菜碱含量测定方法的建立和提取方法的优化[J]. 中国药房,2020,31(14):1700-1703.
[57] Adams M, Wiedenmann M, Tititel G, et al.HPLC-MS Trace Analysis of atRopine in Lycium barbarum Berries[J]. Phytochemical Analysis,2006,17(5):279-283.
[58] Zhao W, Shi Y.Simultaneous Quantification of Three Tropane Alkaloids in Goji Berries by Cleanup of the Graphene/Hexagonal Boron Nitride Hybrids and Ultra-high-perFormance Liquid Chromatography Tandem Mass Spectrometry[J]. Journal of Separation Science,2020,43(18):3636-3645.
[59] Chen H, Kong JB, Zhang L, et al.Lycibarbarines A-C, Three Tetrahydroquinoline Alkaloids Possessing a Spiro-Heterocycle Moiety from the Fruits of Lycium barbarum[J]. Org Lett,2021,23(3):858-862.
[60] Wang K, Sasaki T, Li W, et al.Two Novel Steroidal Alkaloid Glycosides from the Seeds of Lycium barbarum[J]. 2011, 8(12):2277-2284.
[61] Forino M, Tartaglione L, Dell’Aversano C, et al. NMR-based IdentifiCation of the Phenolic Profile of Fruits of Lycium barbarum(goji berries). Isolation And Structural Determination of a Novel N-feruloyl Tyramine Dimer as The Most Abundant Antioxidant Polyphenol of Goji Berries[J]. Food Chemistry,2016,194:1254-1259.
[62] Gao K, Ma D, Cheng Y, et al.Three New Dimers and Two Monomers of Phenolic Amides from the Fruits of Lycium barbarum and Their Antioxidant Activities[J]. Journal of Agricultural and Food Chemistry,2015,63(4): 1067-1075.
[63] 徐秀珍,齐喜红. 薄层扫描法测定枸杞子中甜菜碱的含量[J]. 中成药,2005(8):941-943.
[64] 赵丽莉,李爱华,辛国省,等. 微波密闭消解ICP-AES法测定宁夏枸杞中矿物元素[J]. 安徽农业科学, 2012,40(5):2648-2650.
[65] 李继成,陈勇夫,李纪霞,等. 宁夏枸杞子中氨基酸和微量元素含量测定[J]. 河南医科大学学报,1992 (4):346-347.
[66] 黄璐琦,詹志来,郭兰萍,等. 中药材商品规格等级通则:第一辑[M]. 北京:中国中医药出版社,2021.
[67] 中国国家标准化管理委员会. 中华人民共和国国家标准 [S].2014,GB-T 18672-2014.
[68] 中国科学院中国植物志编辑委员会.中国植物志[M]. 北京:科学出版社,1978:8-15.
[69] 林楠. 不同产地枸杞品质对比研究及优良株系的筛选 [D]. 兰州:甘肃农业大学,2013.
[70] 王莹,时锐,张南平,等. 不同产地和不同品种枸杞子中多糖成份测定[J]. 中国现代中药,2022(15): 165-168.
[71] 张波,罗青,王学琴,等. 不同产区宁夏枸杞品质分析比较[J]. 北方园艺,2014(15):165-168.
[72] 李越鲲,米佳,闫亚美,等. 不同产地宁夏枸杞主要化学成分分析[J]. 食品工业科技,2017,38(21): 286-288,329.
[73] 董静洲,杨俊军,王瑛. 我国枸杞属物种资源及国内外研究进展[J]. 中国中药杂志,2008(18):2020-2027.
[74] 王琪,唐惠儒. 黑果枸杞和宁夏枸杞果实中多酚类物质组成的差异研究[J]. 上海交通大学学报(医学版), 2019,39(1):39-46.
[75] 岳媛,周红,徐云倩,等. 不同等级及产地黑果枸杞原花青素含量比较[J]. 时珍国医国药,2019,30(10):2489-2490.
[76] Rajkowska K, Otlewska A, Broncel N, et al.Microbial Diversity and Bioactive Compounds in Dried Lycium barbarum Fruits(Goji):A Comparative Study[J]. Molecules,2023,28(10):4058.
[77] 张自萍,史晓文,曹丽华,等. 枸杞品质及其与土壤肥力关系的研究[J]. 中草药,2008(8):1238-1242.
[78] 蔡国军,张广忠,张宝琳,等. 氮、磷、钾对枸杞果实糖类、胡萝卜素含量的影响[J]. 西南农业学报, 2013,26(1):209-212.
[79] 尹亮,尹娟,赵彦波,等. 旱区水肥耦合对枸杞果实糖类和类胡萝卜素的影响[J]. 灌溉排水学报,2018,37(8):27-31.
[80] 郑国保,马玲,撖志明,等. 不同生育期水分胁迫条件下宁夏枸杞果实的主成分分析与综合评价[J]. 节水灌溉,2022(1):47-51,57.
[81] 徐青,郑国琦. 不同灌溉方式对宁夏枸杞果实主要品质的影响[J]. 江苏农业科学,2009(6):256-258.
[82] 高以丹,胡娜,王洪伦. 基于GC-IMS比较不同干燥方式对柴达木枸杞中挥发性物质的影响[J]. 天然产物研究与开发,2022,34(12):1989-1998,2033.
[83] 任贵平,王晓雨,程竹林,等. 基于变异系数法对不同温度干燥枸杞品质的评价[J]. 现代食品科技,2022, 38(12):264-271.
[84] 赵丹丹,陈冬,彭郁,等. 枸杞热风干燥过程动力学模型及品质分析[J]. 中国食品学报,2018,18(3): 114-124.
[85] 杜芷茵,王玉莲,万欣雨,等. 贮藏温度和湿度条件对茶叶、干贝和枸杞贮藏期间品质变化的影响[J]. 食品科技,2023,48(5):34-41.
[86] 程竹林. 干枸杞贮藏期间颜色劣变原因与品质控制的研究[D]. 乌鲁木齐:新疆农业大学,2022.
[87] 程竹林,王晓雨,任贵平,等. 电子束辐照对干枸杞生虫及品质的影响[J]. 现代食品科技,2023,39(2): 221-228.
[88] 钱秀玉,聂黎行,戴忠,等. 中药质量等级评价研究进展[J]. 药物分析杂志,2019,39(10): 1724-1737.
[89] 李欣然,康靖杰,夏林波,等. AHP-CRITIC混合加权评分法对辽宁产白鲜皮质量评价[J]. 广州化工, 2022,50(21):127-129,154.
[90] 王艳平,崔永蕾,黄丽,等. 基于灰色关联度法的枸杞子质量评价[J]. 中国现代中药,2020,22(11): 1851-1856,1862.
[91] 李艳萍,刘冰,刘楠,等. 不同种质宁夏枸杞红外光谱特征及其与表型性状的相关性分析[J]. 食品研究与开发,2023,44(9): 178-182.
[92] 王丹,张久旭,范晶,等. 基于图像处理技术的枸杞子商品规格等级评价方法研究[J]. 世界科学技术-中医药现代化,2020,22(8):2817-2823.
[93] 郭金玉,张忠彬,孙庆云. 层次分析法的研究与应用[J]. 中国安全科学学报,2008,18(5):148-153.
[94] 赵建华,述小英,李浩霞,等. 不同果色枸杞鲜果品质性状分析及综合评价[J]. 中国农业科学,2017,50(12): 2338-2348.
[95] 白光灿,李娅琦,张泽坤,等. 采用HPLC指纹图谱技术及数据分析方法对不同产地枸杞进行质量评价研究[J]. 中南药学,2018,16(6):725-730.
[96] 周凯,邬学军,宋军全,等. 数学建模[M]. 杭州:浙江大学出版社,2012:436.
[97] 郭晓晗. 中药鹿茸的真伪鉴定及鹿茸饮片的质量等级评价方法研究[D]. 北京:中国食品药品检定研究院, 2019.
[98] 乔亚玲,宋霞,刘亚蓉,等. 基于UPLC指纹图谱与抗氧化谱效关系的枸杞子质量标志物研究[J]. 中国现代中药:1-12.
[99] 周永峰. 基于商品规格等级和效应成分指数的五味子质量评价研究[D]. 昆明:昆明理工大学,2018.
[100] 李阳. 基于电子鼻技术的厚朴商品规格等级标准研究 [D]. 北京:北京中医药大学,2018.
[101] 崔丹丹. 穿心莲药材及饮片质量等级评价研究[D]. 广东:广东药科大学,2020 .
[102] Wang Y, Wang Z, Zhang J, et al.Evaluation of the Quality of Codonopsis Radix in Different Growth Years by the AHP-CRITIC Method[J]. Chemistry & Biodiversity, 2023,20(6): e202201108.
[103] 李力,潘倩雯,刘宏. 灰色关联度分析法在中药谱效学研究中的应用[J]. 中国药房,2018,29(11): 1581-1584.
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