目的: 对5批进口紫草的质量进行比较研究,为进口紫草的质量评价提供参考依据。方法: 分别对5批进口紫草性状进行对比;采用高效液相色谱法,Venusil MP C₁₈（4.6 mm×250 mm,5 μm）色谱柱,以乙腈-0.05%甲酸水溶液（70 ∶ 30）为流动相,检测波长275 nm,柱温30 ℃,流速1.0 mL · min^-1,对5批进口紫草中乙酰紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁和异戊酰紫草素4个成分进行含量测定。结果: 5批进口紫草在性状上存在较大差异;乙酰紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁、异戊酰紫草素进样量分别在0.2024~2.024 μg（r=1）、0.04072~0.4072 μg（r=0.9998）、0.2062~2.062 μg（r=1）、0.2678~2.678 μg（r=1）范围内呈现良好线性关系;平均加样回收率101.2%~104.1%,RSD<2.4%;不同批次的进口紫草乙酰紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰阿卡宁、异戊酰紫草素含量范围分别为0.030%~0.52%、0.029%~0.43%、0.19%~2.02%、0.39%~1.30%。结论: 进口紫草性状外观形态和内部化学成分有一定关系,建立的含量测定方法简便、准确、重复性好,可为进口紫草药材多指标成分的质量评价和控制提供依据。

Objective：To compare the quality of 5 batches of imported Arnebiae Radix, provide a reference for the quality evaluation of imported Arnebiae Radix. Methods：The characteristics of five batches of imported Arnebiae Radix were compared. The contents of acetylshikonin, isobutyrylshikonin, β,β’- dimethylacrylalkannin, and isovalerylshikonin in five batches of imported Arnebiae Radix were determined by high performance liquid chromatography (HPLC) performed on a Venusil MP C₁₈ column (4.6 mm × 250 mm, 5 μm), with a mobile phase of acetonitrile-0.05% formic acid water (70 ∶ 30) at a flow rate of 1.0 mL · min^-1, ultraviolet detection at 275 nm, and column temperature at 30 ℃. Results：There were significant differences in the characteristics of the five batches of imported Arnebiae Radix. Good linear relationships were observed for acetylshikonin, isobutyrylshikonin, β,β’- dimethylacrylalkannin, and isovalerylshikonin within the ranges of 0.2024-2.024 μg(r=1), 0.04072-0.4072 μg(r=0.9998), 0.2062-2.062 μg(r=1), 0.2678-2.678 μg(r=1), respectively. The average recovery rate were 101.2% to 104.1% with RSD<2.4%. The contents of acetylshikonin, isobutyrylshikonin, β,β’- dimethylacrylalkannin, and isovalerylshikonin in the five batches of imported Arnebiae Radix were 0.030%-0.52%, 0.029%-0.43%, 0.19%-2.02%, 0.39%-1.30%, respectively. Conclusion：There is a certain relationship between the appearance and internal chemical components of the imported Arnebiae Radix. The established content determination method is simple, accurate, and has good repeatability, which can provide a basis for the quality evaluation and control of multi-index components of imported Arnebiae Radix.